The Principles of Beneficence and Nonmaleficence Assignment

The Principles of Beneficence and Nonmaleficence - Assignment Example The treatment that could be offered to her is radiation and chemotherapy, which are not the solutions or cures for the disease but only extending the life cycle of the patient. Therefore, the goals of the treatment are not concerned with the reversal of the health conditions, but for the preservation of life to the maximum extent possible. In totality, the patient cannot really escape the fact that her life is going to end shortly, maybe within the next few years. Medication is a method of prolonging her life, to a certain extent and not complete restoration, in her case. The patient is evidently, well informed and capable in terms of mobility and mental conditions. She is completely competent, because of the facts that she had worked in the medical field previously and also since she had noticed the mass and the severe pain and had taken the initiative to approach a professional, rather than perhaps subsiding it. The patient confesses that she suspects she is a victim of cancer and therefore, asks the medical student to tell her the truth. She is also aware of the fact that the doctors might, after all, not inform her about the disease and diagnosis, in case it is cancer. The patient has not yet been informed about the disease and she is yet to receive the confirmation of her apprehension that she has cancer. The very fact that the patient quite willingly and readily agreed to undergo the surgery, without posing a problem conveys that the patient was cooperative and trusted her doctors. However, on the whole, we see that the patient's desire to be informed and share the knowledge of the doctors, regarding her condition is in all probability not met with. Therefore, one can draw the conclusion that the ethics and laws governing medicine have not been met with. Had the patient been an ignorant and non-cooperative person, the best alternative could be giving treatment and keeping the patient in the dark. But since the patient is from a medical background herself and is well aware of the situation, it is most ethical to inform her about it and give treatment with mutual cooperation.

S there a genuine right to have children, and if so how far does it Term Paper

S there a genuine right to have children, and if so how far does it extend Explore either the question of an individual r - Term Paper Example This paper is a critical evaluation of the human’s natural right to have children as a comparative debate on different socio-economic and political scenarios of different countries. Genuineness of Right to Have Children This question is, perhaps, the most personal one for most people. Having a child is the most basic reason behind legitimate relationships between male and female by form of marriage in all the demographic groups. It is a subject under the religious and cultural jurisdiction of the mankind. Many societies, even if it is a part of an overpopulated country, have a greater speculation on the outcome of a marriage and they think it is the most basic success signal of an adult’s life. According to some suggestions, â€Å"associated with the right to marry is the right to have children, if one chooses, without arbitrary governmental interference.† (Keetley &Pettergrew 212). Since the term ‘marriage’ is a closer associate of religious and cul tural fashions of people, the need for respecting the expectations about marriage becomes a responsibility of the couple. In such a scenario, the need for relating this very question to the social and religious norms of civilized communities across the world is of prime importance. An exploration into the argument to support birth control policies and individual rights to decide the number of children should travel through the studies of different countries and cultural groups besides the influence of many ethical and religious factors facing the human life. Moreover, the entire world today is focusing on the reservations on unprepared pregnancies by adopting medical and spiritual measures to curb the problem of unwanted births for the safety of dignity of even the unborn children from being thrown to desperate living conditions owing to the present day social conditions. Social implications of birth rate rise According to Duane, from the political point of view, the standardization of the population of a country by limiting the birth rate is meant for its economic sustainability and the retained capability to provide educational and employment opportunities to its citizens. There are evidences of some developments in the field of classified breeding for a better generation in countries like America. If scientific approach to quality control of human community is an option for some countries, strict regulations on limiting the birth rate is a choice for countries like China, whose single child policy is a strong example of the government’s intervention in the individual choice of having children. However, there are drastic effects on the social balance when certain governments implement such policies. Situations in some countries worsen in the spiritual and social prospects along with the increasingly biased approach of parents towards girl children. Most people prefer a choice of male children for their assumed financial and social security concerns to girls by indirectly causing a dip in the sex ratio. From a humanistic perspective, it is the right endorsed to every individual to have the natural right to reproduce. All the developments in the world today are the result of such passages of generations.

Child rights based juvenile justice system in Bangladesh

Child rights based juvenile justice system in Bangladesh Juvenile delinquency is a burning issue and relatively a new phenomenon in the criminal and human rights jurisprudence. The juvenile of the world are innocent, vulnerable, and curious, full of hope, and belongs to thrill, aspirations, ambition and what not. In face the future of the juvenile should be shared and cared in the light of equality, dignity and social justice with a view to protecting and promoting the best interest of the child in general. The situation of juvenile delinquency in Bangladesh is very critical in nature. The Convention on the Rights of the Child and other relevant international standards for dealing with juvenile offenders provides a rough but broadly adequate framework for any national system. However, Bangladesh is lagging behind in implementing international juvenile justice standards and the domestic legal initiatives for the protection of juvenile offenders are not adequate somehow and to some extent, these are not consistent with the spirit of the Conv ention of the Rights of Child. Next to non-implementation of laws, the mindset and approach of the people to child offenders are crucial factors that require attention. This essay at the outset, considers the implications of genuine protection of the rights of juvenile offenders. It also discusses some dilemmas for national legislation and the impact of the relevant international instruments on such legislation. Finally the present study has been carried to find out how a child rights-based approach can advance to provide true dignity and justice for child offenders in Bangladesh. Historical background of juvenile delinquency: Delinquency was available throughout all the time. In ancient Britain, even seven years old boys were tried, convicted and punished as adults. There was no special treatment for them, a hanging was a hanging. In Norway, for example, a thirteen century penal code specifies that adult might lose both hands if stealing, children only one (The Council of Europe,2004:13). However, United States of America is one of the pioneer countries on juvenile justice. Social reformers started to create special facilities for troubled juveniles in the late nineteenth century. Though Illinois introduced a separate system of criminal justice in 1899, much had changed by 1909 when Judge Julian Mack famously proposed in a Harvard Law Review article that a juvenile offender should be treated as a wise and merciful father handles his own child (Scott and Laurence, 2008:16). Yet the provision of special court and treatment was soon replicated throughout the country and spread aboard in Britain(1908), France (1912), Spain(1918), The Netherlands(1921)(The Innocenti Digest, 1997:10) Today most of the states of the United Nations have a separate court system for the children accused of an offence. They have the right to treatment that takes full account of their age, circumstance and their needs. The courts generally handle two types of juvenile offender: The delinquent child who has committed an act that is a crime for adult under state law. On the other hand, status offenders are considered unruly and uncontrolled beyond their legal guardian (Siegel and Welsh, 2008). A multitude factors exist that contribute to the understanding of what leads to engage in delinquent behavior. It is also true certainly social factors like poverty, illiteracy, broken homes; lack of employment, peer pressure, lack of parental guidance can be the best root causes to explain juvenile delinquency in Bangladesh. In Bangladesh no separate juvenile justice law exists. The 1974 Children Act is the main law concerning children in Bangladesh, but it deals both with children in need of protection/care as well as children in conflict with the law on one hand, other laws, such as the Penal Code, the Code of Criminal Procedure, the Suppression of Violence against Women and Children Act, on the other hand, also contain provisions regarding children in conflict with the law (UNICEF, n.d.).At present, there are three specialized juvenile courts established in the country and three specialized institutions for the detention of child offenders, which have been renamed as the Child Development Centers among them, two centers for boys are located in Tongi and Jessore and the girls centre is in Konabari (ibid) Definition of a juvenile in Bangladesh: contradictions and dilemmas The definition of child is not uniform in the laws of Bangladesh. Different laws have defined children in different ways. According to the section 2(f) of the Children Act 1974 defines a child as a person under the age of sixteen for the purpose of juvenile justice system administration. Nonetheless, UN Convention on the Rights of Children defines as any person under the age of 18 years. In addition the Bengal Vagrancy Act in section 1(3) explains a child as a person less than 14 years. According to the Women and Children Repression Prevention (Special Provisions) Act, 2000 amended in 2003 furthermore, defines a child as a person under the age of 16 years It is indeed to say that as far as proper functioning of the juvenile justice system is concerned the determination of age is extremely important. However in regard to criminal offence committed by a child, the laws in Bangladesh are to some extent ambiguous. To determine the age of criminal responsibility section 82 of the Penal Code 1860 says that nothing would be an offence, which is done by a child under nine. Surprisingly in the same statute section 83 provides, nothing is an offence which is done by a child above seven years of age and under twelve who has attained sufficient maturity to understand the nature and consequence of the act'(Ministry of Law and Parliamentary Affairs, Government of the Peoples Republic of Bangladesh 1860). So it is one of the difficulties of criminal justice policy to provide the legal mechanism to reflect the transition from the age of childhood innocence and through the maturity and the full responsibility under the criminal law. The article 40 CRC asks the states to establish a minimum age below which children shall be presumed not to have the capacity to infringe the penal law (Convention on the Rights of the Child, 1989). The treaty does not spell out at which precise age the line should be drawn though committee monitoring the implementation of the Convention has expressed the concern about the low age in several countries (Hammarberg 2008:194). Rule 4.1 of the Beijing Rules provides, in those legal systems recognizing the concept of the age of criminal responsibility for juveniles, the beginning of that age shall not be fixed at too low an age level, bearing in mind the facts of emotional, mental and intellectual maturity (United Nations Standard Minimum Rules for the Administration of Juvenile Justice, 1985). These international standards unfortunately are not followed in the courts of Bangladesh as there is no uniformity in the definition of a child. In concern with this the Committee on the Rights of the Child in its concluding observations of 2003 expressed over the limited progress achieved in establishing a functioning juvenile justice system throughout the country(UNICEF, n.d.). International legal framework for a child rights oriented juvenile justice: The major international conventions dealing with juvenile justice secures the protection of a child in conflict with law and urges for special action in accordance with the nature and circumstance of the offence. Juvenile justice has become an international issue with the UNCRC coming into force in 1990. Articles 37 clearly states, No child shall be subjected to torture or other cruel, inhuman or degrading treatment or punishmentEvery child deprived of liberty shall be treated with humanity and respect for the inherent dignity of the human person, and in a manner which takes into account the needs of persons of his or her age (Convention on the Rights of the Child, 1989). In addition, article 40 connotes, States Parties recognize the right of every child accused of infringing the penal law shall be treated in a manner consistent with the promotion of the childs sense of dignity and worth, which takes into account the childs age and the desirability of promoting the childs reintegration in society (ibid). These special provisions also focus on several rights like, right to be informed, rights to a fair trial, right to legal assistance and so forth. This convention is binding legally on Banglad esh and operates as hard international law. Apart from CRC, there are three other important UN sponsored documents dealing explicitly with the standards and guidelines for the treatment of children coming with the conflict of law. The Beijing Rules conveys the basic procedural safeguards for a juvenile offender, such as the presumption of innocence, the right to be notified of the charges, the right to remain silent, the right to counsel, the right to the presence of a parent or guardian, the right to confront and cross-examine witnesses and the right to appeal to a higher authority (United Nations Standard Minimum Rules for the Administration of Juvenile Justice, 1985). Riyadh Guidelines moreover, call for a child centered orientation and child development perspective to prevent delinquency that directly refers to adoption of the preventive policies facilitating the successful socialization and integration of all children and young persons, in particular through the family, the community, peer groups, schools, vocational trai ning and the world of work as well as through voluntary organizations (Krech,1998), whereas the other charter urges for a juvenile justice system that should uphold the rights and safety and promote the physical and mental well-being of juveniles (UN Rules for the Protection of Juveniles Deprived of Liberty, 1990). Besides, there are additional documents which are often referred to in any discussion on Juvenile Justice, for instance, Standard Minimum Rules for the Treatment of Prisoners of 1955, UN standard Minimum Rules for Non-custodial Measures of 1999 etc. It is important to note that Bangladesh is not a signatory party of these international instruments for juvenile justice, but it is morally bound with the provision of these guidelines and directions for the betterment of the juvenile. . Human rights violation of the juveniles in conflict with law in Bangladesh: In Bangladesh, according to the Children Act 1974 and also the Code of Criminal Procedure, children can only tried by juvenile court and no trial can be held with the adults. The trial shall be held in camera i.e. only people directly involved in the case and officer of the courts can be present during the trial. Even the report of the probation officer or any other report considered by the court shall be treated as confidential and publication of report of the proceedings, photograph of the child leading directly and indirectly the identity of such child is prohibited. But these provisions have been rarely followed in trying a juvenile delinquent. The juvenile justice system in the country remains in a chaotic situation as there are dozens of judgments convicting the youth offenders under the Children Act 1974, although there cannot be any punishment to children under 16(The Daily Star,2007) If it appears to the court the accused is a child, the court shall make an inquiry to determine the age of the child. In the absence of birth registration practice, the judicial officers have to depend on mere speculation or, medical certificates of the child. Section 48 states that if a child arrested is under 16 years old, the police officer may release him on bail (The Children Act 1974). But the reality tells something else. A study by Odhikar it was found that in 2001 in Dhaka Central Jail 7% of the children arrested with adults on suspicion under section 54 of the Code of the Criminal procedure 1898 had been in custody for more than 2 years (Chowdhury, 2006). In 2002, a 14year old boy was released from prison in December after two and half year in custody. The child was arrested in a blanket sweep against criminals and never charged with any offence (US Bureau of Democracy, Human Rights, and Labor, 2004).In July, 2005 the Dhaka Metropolitan Magistrate Court released a nine year s old boy from criminal case after investigators from a local human rights organization noticed him during a regular prison visit (US Bureau of Democracy, Human Rights, and Labor, 2005). After the arrest of the child, neither the probation officers are informed by the police nor are the guardians found with the help of probation officers. For the protection of best interest no child would be tried, detained with adults and sentenced with life imprisonment. Nonetheless, the issue of joint trial of a child with an adult has been in a number of cases. For example, in Shiplu and another vs. State (1997), Shiplu a boy of 14 years old was tried with his mother for killing a woman under section 302 under the Penal Code 1860. They were convicted with the District Court and sentenced to imprisonment for life. In the appeal preferred by Shiplu, the conviction of the trial court was invoked by appellants advocate to be set aside for two reasons, Shiplu was a child at the time of the trial and the trial of him was not taken place in a juvenile court. Therefore the Appellate court accepted the disposition of the appellants lawyer and issued an order that the conviction as given b y the District court would be set aside. In reference with this, a report shows approximately 347 children were imprisoned (some with their mothers) despite laws prohibiting the imprisonment of minors (US Bureau of Democracy, Human Rights, and Labor, 2009) The governments human rights record remained poor, and the government appeared to be inactive to assist the rights of the child offender. Even other concerned authorities including the judges of the court seem to be very careless to take into account the domestic laws of Bangladesh. In 2004, a 3-year-old child, accused in an attempted murder case, surrendered before Court of Chief Metropolitan Magistrate and prayed for bail (The Daily Star,2004). A research conducted on ten child offender sentenced under the Penal code in Bangladesh shows that the youthful offenders were kept in the jail custody with the adult prisoners and most of them were detained in the District Jail not in the child development centers, even no probation officer visited them during the pendency of the legal procedures (Malik, 2007:41-42) If a child has been kept in the child development centre, he can exercise his rights to education, leisure; participate in training, cultural activities and so on, unfortunately poor administrative and logistic support lead to a hindrance to protect the best interest of the child in such centers. The probation officers dont submit the report to assess the improvement of children regularly and the inmates are not produced on the scheduled day before the court (Khan and Rahman, 2008:83-84) As Bangladesh is one of the state parties of the CRC, it is obligatory for the state to undertake all appropriate legislative, administrative, and other measures for the implementation of the rights of the children in conflict with law. The child rights oriented juvenile system does fit to a country like Bangladesh to fulfill, protect and promote the rights of the children. Though there are some challenges still remaining, I would like to argue that child rights based juvenile justice framework would be very reliable to explore youth justice in Bangladesh through transparent use of resources, CRC centered policies and the collaboration of the actors. What is a child rights based juvenile justice system: The initial idea behind a juvenile justice system is that children in trouble with the law should be helped rather than punished. Central to the concept of the juvenile court was the principle of parens patriae. This means that instead of lawyers fighting to decide guilt or innocence the court would act as parent or guardian interested in helping the child (Family law,n.d.). The child rights based juvenile justice is a holistic approach basically prioritizes the principles of non discrimination and equality (article 2, CRC), well being of the child (Article 3, CRC), and participation, voice and volunteerism (article 12, CRC). It implies the progressive realization of the rights of the juvenile offender through the separate judicial mechanism and special treatment with a view to facilitating human dignity and integrity. It hold the powerful accountable to come up with the child centered legislations and policies and allocate budget and more resources for the delinquent. The child rights based juvenile justice seeks to improve the quality, responsiveness and relevance of the institutions and services like child oriented judiciary and probation office, well equipped correctional centers etc. Besides, a child friendly juvenile justice framework intends to change awareness, attitudes, behavior, practices, norms, and values in the society by the sensitization and training of different sections. However a question can come into play whether the offender would go unpunished in this framework. The procedures under a child rights based approach may recognize the damage to the victim and it should make the juvenile offender understand that the deed was not acceptable (Hammarberg 2008:194). But the main difference with the ordinary criminal justice is that child rights juvenile system doesnt advocate for retributive procedure and encourages to establish responsibility of the actors to promote rehabilitation and re-integration (ibid). Tools of structuring rights based juvenile justice system: Child rights juvenile justice needs a wide range of tools to achieve concrete and sustainable results for children wellbeing. It works to get duty bearer to fulfill their obligation and to support children to claim their rights. The State is required to render appropriate assistance additionally to parents and legal guardians in the performance of their child-rearing responsibilities, thus ensuring that the capacity of families to act as the first line of protection of children is enhanced (Pais, 1999:13). Initiating legal reforms: Necessary amendments in regard to the national juvenile laws need to be brought into the line with the standard laid down by CRC. For instance, under the Children Act urgent reforms should be made to consider everyone below the age of eighteen years (Khan and Rahman, 2008:103). In Uganda, specialized family and children courts with informal settings and speedy trial procedure have been established for rehabilitating the children in the society (Roy and Wong, 2006:45).In Bangladesh the national legislation doesnt recognize the principle of diversion that needs to be maintained with the provision of community based rehabilitation, NGOs involvement, etc, even it does not urge for the establishment of a child ombudsman office. These legal reforms are in the core of child rights based youth justice. However, Bangladesh government started to concentrate to a new national social policy on models of care and protection for children in conflict with the law has been drafted to address both ch ildren in conflict with the law and children in children of protection (Ahmed and Islam, 2010:281). Providing adequate legal aid: Most of the children who come in conflict with law in Bangladesh come from very poor families who dont have the ability to engage counsel for their defense. Article 40, UNCRC notes that he child is entitled to have legal or other appropriate assistance in the preparation and presentation to defend him. In accordance with the convention the strategy under child rights model accounts the government to provide free legal assistance to the vulnerable children to ensure the fair and prompt trial. Child friendly Court environment: In Bangladesh, in the children development centres there are separate courts for children which are in comparison to other criminal courts more favorable for the children. But very few cases are heard there. Majority of the cases tries in the ordinary criminal court with adverse court environment and the required special procedure specified in the Children Act is not followed. The child is not heard and he is not allowed to participate in the court proceedings as well. The child rights oriented court system identifies the duty of police, judge, and public prosecutor and broadly the governments by allocating resources and providing trained actors(judges, police etc) to compose an informal court environment that secures the participation of the children and secondly promotes the establishment of laws, procedure in favor them in coordination with article 40(3), CRC. Serving facilities in the juvenile development centers: The centers in Bangladesh lack facilities for correctional activities. The curriculum for the vocational training is not up to date, the instructors are not skilled enough and the officials lack skill to deal with the children who need special care and attention. One 10year old boy was found killed in the Juvenile Development Centre with his throat slit and another one reportedly died in a clash between prisoners'(Odhikar,2008:2). In this context, the juvenile rights based youth justice demands to incorporate counseling, modern trade for the correction centres and arrangement of training for the centre employees including the probation officer. Duty of the NGOs, INGOs and Social Welfare Departments: The NGOs with shelter home can secure the protection of a child in conflict with law. They have the duty to provide free legal aid and act on behalf of a juvenile. In Vietnam Save the Children (Sweden) approached by the Ministry of Justice for technical assistance developed the capacity of staff in various relevant agencies like police, prosecutors, judge and the officials of mass organization and initiated programs to ensure familiarity with UNCRC, international standard, divergence between police policy and practice on the ground(Roy and Wong,2006:76).The new child rights concept involves a shift to justice and entitlement of the children and acknowledges immediate survival and development of them by making the duty holder accountable. So, the probation officers under the Social Welfare Department are bound to exercise their power and perform their duties properly to train the offender and rehabilitate them in society. Necessity of training and sensitization: Protection of the best interest of the child by community service: The Beijing Rules specify care, guidance, probation services, and community services as non custodial sentencing options in section 18. In accordance with the international norms, community service asks the child to provide service to the community as means of punishment or restitution and allows juveniles to remain with their families. The rights focused juvenile justice can address the community service in Bangladesh as a meaningful method in which young people can see the benefits of their works Inter-agency collaboration: Children justice plannings in Bangladesh seek the involvement of a wide range of people from various institutions, government department and the society including judiciary, police, probation officer, stakeholders, community leaders, social welfare personnel, staff of UNICEF to promote the integration of a child offender. International guidelines emphasis the importance of inter ministerial and inter departmental coordination to improve the quality of institutional treatment of the children as well (section 26, The Beijing Rule and section 60, Riyadh Guidelines) Challenges to develop a child rights based juvenile justice model in Bangladesh: To me, it is relative early stage to build up a child focused juvenile justice system successfully. Ideologically it is associated with neo-liberal agenda and in Bangladesh context; some encounters may hinder the process of the development of rights based approach to youth justice in Bangladesh. Lack of available resources: This approach binds the government with their effective efforts to promote the rights of a child offender inside and outside the court by the maximum use of resource. Establishing well functioning juvenile courts and national council for children, effective mechanism for birth registration programs and other promotional activities demand a high profile budget which may slow down the process of child development. Absence of coordination among stakeholders: In Bangladesh different stakeholders have been carrying out many activities in the area of pro child manner juvenile justice. Though the enthusiasm is high, these activities have often been proved to be sporadic in nature due to the lack of coordination among them. Deficiency in monitoring role of authorities: In the courts of Bangladesh there is no provision as well as facilities to monitor the progress of the dispositional orders. Thats why when the child is placed out at home, it becomes almost impossible to supervise his condition whether he gets involved with another offence or he is able to enjoy his rights. Lack of political will or commitment of the duty bearers with limited public accountability: I like to conclude that there is a light of hope that during the recent years government and other NGOs have been working hard to improve the human rights condition of the child offender. The juvenile correctional centres, which were, in practical terms, jails for young boys allegedly involved in criminal activities, were transformed into child development centres that tried to promote more humane approaches towards treating the children in the centres and to acknowledge their role as active citizens( Judge,2007:28).. However the government intends to consider those children who are detained in jail, not the children who have been back in their families (Khan and Rahman, 2008:103). However the rights oriented approach does not allow separation among the children and reveals to aid the best interest of child regardless of their birthplace or status Therefore I like to reckon that it is an approach that can be successfully filtered to inspire, persuade and support states in discharging their obligation to juvenile offenders rights in Bangladesh.

Alcoholism :: essays research papers

Alcohol is the intoxicating part of beer, wine and liquors-the part that causes drunkenness. It is formed during fermentation, the process that creates the alcohlolicbeverage. When sugars from the fruits or grains are combined with yeast and water, alcohol results. Alcohol is a drug and, like all drugs, it has an effect on a person’s body and mind. Because drinking alcoholic beverages makes some people feel more alive and more outgoing, alcohol is sometimes seen as a stimulant. But in fact it is a depressant, and slows down the central nervous system, of which the brain is a part. Small amounts of alcohol can affect a person’s coordination and judgment. Drinking a large amount of alcohol at one time can even cause death. Alcohol is estimated to be contributing factor in 20-30% of all accidents. In fatal car accidents involving young men after 10pm it is a contributory factor in 60% of these cases. About 30% of all drowning are estimated to be alcohol related. This propo rtion may rise to 50% between the ages 20-30. Alcohol is also a poisonous. It must be broken down and removed from the body. However, it leaves behind toxins, or poisons, that can cause health problems and contribute to serious diseases. Beer contains the least amount of alcohol, about 3-6%. Wine is 8-14 percent alcohol. Distilled spirits have a much higher alcoholic content. The alcoholic content of gin, scotch, vodka, whiskey, rum, and bourbon is about 40%. When alcohol enters the body this is what happens. Within 20 minutes of entering the stomach, as much as 20% of the alcohol in a drink is absorbed into the bloodstream. The rest remains in the stomach where it stimulates the secretion of gastric juices. Large amounts of alcohol entering an empty stomach can irritate the gastric lining and cause the stomach to become inflamed. From the stomach, the alcohol passes into the small intestine. Here the rest of it is absorbed through the intestinal wall into the bloodstream. From the bloodstream, about 5 percent of alcohol leave the body unchanged through urine, sweat, or exhaled breath. Next the alcohol travels via the bloodstream to the heart. Small amounts of alcohol produce a slight increase in heart rate and blood pressure. Larger amounts reduce pumping power of the heart and can cause an irregular heartbeat. The heart then pumps the alcohol through the blood vessels to other parts of the body, including the brain.

Principles of personal devlpment Essay

Data protection act Freedom of information act 1995 Aii) Human rights act Disability discrimination act 1995 Aiii) Human rights act Control of substances hazardous to health regulations 2002 Aiv) Care standards act 2000 Code of practice Av) Safeguarding vulnerable adults groups act 2006 Policies and procedures Avi) A PDP is a personal development plan and is done between you and your manager. They will record all information about your learning and development needs in work. Things like targets, agreed objectives and activities to meet them and standards that are required. These all build your own appraisal. Avii) Carers, members of staff that work with you. The individuals you care for and their family Supervisor, manager. Task B Case study Bi) Jenna must make sure that she respects other people’s beliefs even though they may be different to her own. She must go about this in a non-judgmental way. She should also read through people’s care plans to gain better knowledge of them and their needs. I also think Jenna should put her own beliefs to one side and then discuss with her boss any issues she may have. It is essential for carers to respect each and every individual as an individual, not letting anything affect this. Maybe she should also look back on how she has spoken to the service user and make sure she doesn’t come across as rude or nasty in the future. Bii) It is very important to reflect on your work activities as it’s a very good way to develop skills and make sure you understand everything. It will also give you chance to plan how you are going to behave if the situation happens again. You can make changes and improve the way you work, it is also very important to reflect on your own behaviour and see how it affects others. During these reflections it’s a very good time to find the help and support you may need to better the way you work. E. g. training sessions. When working in care it is important that you learn to think about your own practice in a constructive way. Reflection and evaluation should not undermine your confidence but you should use them in a constructive way to identify areas for improvement. Ci) Moving and handling course – training session Knowing how to safely move a resident e. g. hoist. Skills – risk assessment is needed for each person, which sling is safe etc. do they need a hoist. We need to know the risks because if the manoeuvre is not completed properly this can cause falls and accidents. Cii) During my senior training I received feedback during my senior training, on how to do peg feeds safely and effectively. My first time, I didn’t happen to flush enough water, so my assessor informed me of this, which then improved my skill as a senior to do my job role properly. I then had then knowledge and skills to improve my work role.

EWI competence criteria Essay

Comply with all workplace health, safety and welfare legislation requirements: QCF 641 Communicate with others to establish productive work practices: QCF 642 Moving, handling and storing resources in the workplace: QCF 643 Installing External Wall Insulation In The Workplace: QCF448v2 QCF 448v2: 1.1 Page REF:IEI 7 Interpret and extract information from drawings, specifications, schedules, method statements, risk assessments and manufacturers’ information. QCF 448v2: 1.2 Page REF:IEI 7 Comply with information and/ or instructions derived from risk assessments and method statements. QCF 448v2: 3.1 Page REF:IEI 14a Use personal protective equipment (PPE) and access equipment/ working platforms safely to carry out the activity in accordance with legislation and organisational requirements when installing external wall insulation. QCF 448v2: 4.1 Page REF:IEI 18 Select resources associated with own work in relation to materials, components, fixing, tools and equipment. QCF 448v2: 5.1 Page REF:IEI 24 Protect the work and its surrounding area from damage in accordance with safe working practices and organisational procedures. QCF 448v2: 5.2 Page REF:IEI 24 Minimise damage and maintain a clean work space QCF 448v2: 5.3 Page REF:IEI 24 Dispose of waste in accordance with legislation QCF 448v2: 6.1 Page REF:IEI 27 Demonstrate completion of the work within the allocated time. QCF 448v2: 7.1 Page REF:IEI 29 Demonstrate the following work skills when installing external wall insulation: measuring, marking out, fitting, filling, finishing, positioning and securing. QCF 448v2: 7.2 Page REF:IEI 29 Prepare and install external wall insulation to given working instructions, relating to one of the following :pre finished installation systems/methods :non finished installation systems/methods QCF 448v2: 7.3 Page REF:IEI 29 Safely use and store hand tools, portable power tools and ancillary equipment QCF 448v2: 7.4 Page REF:IEI 29 Safely store the materials, tools and equipment used when installing external wall insulation Applying Surface Finishes To External Wall Insulation In The Workplace: QCF 449v2 QCF 449v2: 1.1 Page REF:ASF 7 Interpret and extract information from drawings, specifications, schedules, method statements, risk assessments and manufacturers’ information. QCF 449v2: 1.2 Page REF:ASF 7 Comply with information and/ or instructions derived from risk assessments and method statements. QCF 449v2: 3.1 Page REF:ASF 14 Use health and safety control equipment and access equipment safely to carry out the activity in accordance with legislation and organisational requirements when applying surface finishes to external wall insulation. QCF 449v2: 4.1 Page REF:ASF 18 Select resources associated with own work in relation to materials, components, fixing, tools and equipment. QCF 449v2: 5.1 Page REF:ASF 24 Protect the work and its surrounding area from damage in accordance with safe working practices and organisational procedures. QCF 449v2: 5.2 Page REF:ASF 24 Minimise damage and maintain a clean work space. QCF 449v2: 5.3 Page REF:ASF 24 Dispose of waste in accordance with legislation. QCF 449v2: 6.1 Page REF:ASF 27 Demonstrate completion of the work within the allocated time. QCF 449v2: 7.1 Page REF:ASF 29 Demonstrate the following work skills when: measuring, marking out, fitting, applying, positioning and securing. QCF 449v2: 7.2 Page REF:ASF 29 Apply finishes to external wall insulation to given working instructions, relating to dash finishes, synthetic renders and one of the following: mortar finishes, proprietary pre cast finishes, paint finishes, corner and surface beads, brick slips, brick effect render, reinforcing mesh. QCF 449v2: 7.3 Page REF:ASF 29 Safely use materials, hand tools, portable power tools and ancillary equipment. QCF 449v2: 7.4 Page REF:ASF 29 Safely store materials, tools and equipment used when applying surface finishes to external insulation.

no title essays

no title essays A Summary of William Raspberrys The Handicap of Definition A Summary of William Raspberrys The Handicap of Definition In The Handicap of Definition William Raspberry emphasizes the stereotype of what it means to be Black. Raspberry uses many beliefs that has taken a toll on African Americans on the definition of being Black. Raspberry uses many comparisons to compare blacks to whites and other ethnics. He begins off by using basketball as an example. Raspberry quotes, If a basketball fan says that Boston Celtics Larry Bird plays black the fan intends it and Bird probably accepts it as a compliment(543). He also emphasizes if you tell a white broadcaster he talks black hell sign up for diction lessons(543). Raspberry explains how many children think hard study and hard work are white (543). He tells how scrimping today in the interest of tomorrows goals is white(543). Raspberry uses many of these comparisons in the essay. Raspberry talks about incredible array of habits done by different ethnic groups. He confesses ...black youngsters tend to do better at basketball, for instance, is that they assume they can learn to do it well, and so they practice constantly to prove themselves right (543). Raspberry emphasizes the importance of developing positive ethnic traditions (544). He tells how people suspect Jews have an innate talent for communications (544). People make assumption that Chinese are born with a gift for mathematical reasoning (544). Raspberry thinks we are ...raising up yet another generation of young blacks who will be failures by definition(544). Raspberry concludes the essay referring to blacks in the work force. He says, many of the things about which blacks make this ...

OIverhead projectors Essays

OIverhead projectors Essays OIverhead projectors Essay OIverhead projectors Essay A resource is any learning aid available to supplement teaching strategies. These range from materials such as books, handout, games, worksheets to audio visual aids like videos, overhead projectors, tape recorders and so on. Other useful resources include objects, samples and models- real life items- that can be brought into the classroom to augment teaching. Trips and outside events can be used to introduce students to the real world . However one really important resource is the students themselves. They come to the classroom with range of skills, know-how and different cultures and their knowledge and past experiences provides a great resource for classroom learning. From the large selection of resources available it is crucial to select a variety of interesting and appropriate resources which support the lesson objectives. One must be careful to use different resources that supports all learning styles of students Below I review some of the resources I have used with my entry 3 literacy students who I taught at Westminister Kingsway college last term and my Entry 2 literacy students who I now teach at Harrow College. The entry 2 students are enrolled with MIND and are all to some extent have mental health problems. Worksheets /Handouts Worksheets are inexpensive to produce. They can be adapted and developed to meet the lesson objective. During the job skills topic that I did with my entry 3 students last term I used a number of worksheets from . I scanned OCRed and enlarged text on a number of occasions as sometimes the page was too crowded or the text too small. The handouts could also be adapted to provide a set of differtiated worksheets to meet the needs of different students. I adapted one worksheet to include more spelling and grammar mistakes for the more able students. Handouts are extremely useful as they also provide an aide memoire for students. However, one must guard against overuse and of giving the student too much paper which may confuse them. Another important point to bear in mind is copyright issues if copying material from books and also to ensure that a wide variety of materials is used where different cultures are valued and recognised. In general as Susan Wallace says handouts must be appropriate to the situation, subject matter and student abilities. Wallace S 2001,p100. I have found that students of all learning styles make good use of worksheets although they are more suited kinaesthetic and visual learners, auditory learners benefit from the discussion around the handout. It is a good idea to display information visually with images where appropriate. As Petty says for many purposes visual information is more effective. Research shows that information enters our brain in the following way.87% by eyes 9%by ears and 4% by other senses (Petty G2001 p 315) On some occasions, the students have use the worksheets in groups first discussing and deciding on the correct answers between them before individually filling in the sheets meeting all learning style. Overhead Projector OHP is a useful resource and there was one in the classroom with my entry 3 students which I used on some occasions. The cost of transparencies are reasonable and they can be reused. The OHP also has the advantage that you can face the class whiles using it thus enabling better classroom management and eye contact with the class.  I have used the OHP on a couple of occasions to sum up the main points of a piece of work as in the Ten top tips for filling out application forms . This emphasised, summarised and reinforced the work the students had been doing on filling out forms. It is necessary to test the OHP before the students arrive. On one occasion it took me quite a while and I needed to get some help from a teacher before I could get the OHP to focus properly. Another thing I learnt was to use very large size fonts as on one occasions I recognise the font was a little small for students at the back of the class to focus properly. After that occasion, any overheads I produced I used at least size 20 font and sometimes larger. I also tried not to put too much text on the acetate and to take note if Myself or the project were obscuring the views of some of the students! The OHP can be a safety hazard with trailing flexes as the plugs can be some distance from the OHP itself. It is important to try to make the environment as safe as possible. I also discovered that it is a good idea to make a handout from the overhead as one the occasion when I didnt the students asked for one which I then had photocopied. Another important point I noted with printing onto transparency from the computer was that it was important to get the right setting, the correct acetate and the correct side. It takes a lot of fiddling about at first. Whiteboard This is a significant resource available in all the classroom at Westminister Kingsway whiteboard is used. I used it a lot for brainstorms, writing down key point, spelling etc. It is important to have several whiteboard markers so if one runs out there are more available and to use the correct type of markers. Using coloured pens to highlight grammar or spelling mistakes is also quite a good idea which I have not as yet utilised fully but intend to use this technique more. The difficulties I have found with the whiteboard are trying to write clearly and in a straight line which was a bit difficult to master. I also have to remember not to talk to the board but to face my students and it is a good idea to look over your shoulder now and then to aid classroom management.

Christmas Trends, Demographics, Spending and Waste

Christmas Trends, Demographics, Spending and Waste Christmas is one of the most widely celebrated holidays by people all over the world, but what are the particularities of it in the United States? Who is celebrating it? How are they doing it? How much are they spending? And how might social differences shape our experience of this holiday? Lets dive in. The Cross-Religion and Secular Popularity of Christmas According to Pew Research Centers December 2013 survey about Christmas, we know that the vast majority of people in the U.S. celebrate the holiday. The survey confirms what most of us know: Christmas is both a religious and a secular holiday. Unsurprisingly, about 96 percent of Christians celebrate  Christmas, as do a whopping 87 percent of people who are not religious. What may surprise you is that people of other faiths do too. According to Pew, 76 percent of Asian-American Buddhists, 73 percent of Hindus, and 32 percent of Jews celebrate Christmas. News reports indicate that some Muslims also celebrate the holiday. Interestingly, the Pew survey found that Christmas is more likely to be a religious holiday for older generations.  While just over a third  of people ages 18-29 celebrate Christmas religiously, 66 percent of those 65 and older do so. For many Millennials, Christmas is a cultural, rather than a religious, holiday. Popular Christmas Traditions and Trends According to the 2014 National Retail Federations (NRF) survey of planned activities for Christmas Day, the most common things we do are visit with family and friends, open gifts, cook a holiday meal, and sit on our bums and watch television. Pews 2013 survey shows that more than half of us will attend church on Christmas Eve or Day, and the organizations 2014 survey shows that eating holiday foods is the activity that we most look forward to, after visiting with family and friends. Leading up to the holiday, the Pew survey found that  the majority of American adults- 65 percent- will send holiday cards, though older adults are more likely than younger adults to do so, and 79 percent of us will put up a Christmas tree, which is slightly more common among higher income earners. Though hurtling through airports at top foot-speed is a popular trope of Christmas movies, in fact, just 5-6 percent of us travel long-distance by air for the holiday, according to the U.S. Department of Transportation. While long-distance travel  increases by 23 percent at Christmas time, most of that travel is by car. Similarly, though images of carolers punctuate holiday films, just 16 percent of us join in the activity, according to Pews 2013 survey Studies also show that we are getting engaged, conceiving children, and deciding to get divorced more so on Christmas than during any other time of  the year. How Gender, Age, and Religion Shape Our Christmas Experiences Interestingly, a 2014 survey by Pew found that religious affiliation, gender,  marital status, and age have an impact on the extent to which people look forward to the  common ways of celebrating Christmas. Those who regularly attend religious services are more enthusiastic on average about Christmas activities than are those who attend less often, or not at all. The only activity that escapes this rule? Americans universally look forward to eating holiday foods. In terms of gender, the survey found that, with the exception of visiting with family and friends, women look forward to the holiday traditions and activities more than men. While the Pew survey did not establish a reason for why this is the case, existing social science suggests that it could be because women spend more time than men do shopping and visiting with or taking care of family members in the context of their everyday lives. Its possible that mundane and taxing chores are more appealing to women when they are surrounded by the Christmas glow. Men, however, find themselves in the position of having to do things that they are not normally expected to do, and so they dont look forward to these events as much as women do. Echoing the fact that Christmas is less of a religious holiday for Millennials than it is for older generations, the 2014 Pew survey results indicate an overall generational shift in how we celebrate the holiday. Americans over the age of 65 are more likely than others to look forward to hearing Christmas music and attending religious services, while those in the younger generations are more likely to look forward to eating holiday foods, exchanging gifts, and decorating their homes. And while the majority of all generations do these things, Millennials are the most likely to buy gifts for others, and the least likely to send Christmas cards (though still a majority do it). ChristmasSpending: Big Picture, Averages, and Trends More than  $665  billion is the amount the NRF forecasts  Americans will spend during November and December 2016- an increase of 3.6 percent over the previous year. So, where will all that money go? Most of it, on average $589, will go  to gifts, out of a total $796 that the average person will spend. The rest will be spent on  holiday items including candy and food (about $100), decorations (about $50), greeting cards and postage, and flowers and potted plants. As part of that decorative budget, we can expect Americans to collectively  spend more than $2.2 billion on about 40 million Christmas trees in 2016 (67 percent real, 33 percent fake), according to  data from the National Christmas Tree Association. In terms of gift-giving plans, the NRF survey shows American adults intend to buy and give the following: Clothing or accessories (61%)Gift cards or certificates (56%)Media items (books, music, videos, games, etc.) (44%)Toys (42%)Food or candy (31%)Consumer electronics (30%)Personal care or beauty items (25%)Jewelry (21%)Home decor or furnishings (20%)Cash (20%)Sporting goods or leisure items (17%) The plans adults have for gifts for children reveal the stronghold that gender stereotypes still have in American culture. The top five toys that people plan to buy for boys include Lego sets, cars and trucks, video games, Hot Wheels, and Star Wars items. For girls, they plan to buy Barbie items, dolls, Shopkins, Hatchimals, and Lego sets. Given that the average person intends to spend nearly $600 on gifts, its not surprising that nearly half of all American adults feel that exchanging gifts leaves them stretched thin financially (according to Pews 2014 survey). More than a third of us feel stressed out by our countrys gift-giving culture, and nearly a quarter of us believe that it is wasteful. The Environmental Impact Have you ever thought about the environmental impact of all this Christmas cheer? The Environmental Protection Agency reports that household waste increases by more than 25 percent between Thanksgiving and New Years Day, which results in an additional 1 million tons per week going to landfills. Gift wrapping and shopping bags amount  to a whopping  4 million tons  of Christmas-related trash. Then theres all the cards, ribbons, product packaging, and trees too. Though we think of it as a time of togetherness, Christmas is also a time of massive waste. When one considers this and the financial and emotional stress of consumerist gift-giving, perhaps a change of tradition is in order?

Home Visit With Sallie Mae Essay Example | Topics and Well Written Essays - 500 words

Home Visit With Sallie Mae - Essay Example Lack of enough oxygen is the main cause of a clouded mind hence the need for the administration of oxygen (Grand Canyon University, 2013). The visiting nurse approached Sallie well, made her feel appreciated and went ahead to assure her that her condition is manageable. To show her concern for what Sallie was going through, the nurse requested her to talk about her late husband. In the medical profession, sharing a patient’s personal problem is important in creating a patient-physician relationship. Like in Sallie’s case where loneliness is one of the stumbling blocks to her recovery, the talk gave her an opportunity to discuss her problems. Efferox was administered at bedtime to treat depression (Grand Canyon University, 2013). The strength of the prescribed drug is an indication that the Sallie was under too much psychological trauma probably because of her medical condition and loneliness To help alleviate the symptoms of congestive heart failure, Sallie was advised to take multivitamins and potassium supplements because she could not provide the body with sufficient quantity of vitamins because of poor appetite. Her poor appetite is also as a result of loneliness because she feels like she has nothing to live for. To relieve her chest pains and headache, she was advised to take ibuprofen and darvocet. Digoxin was administered to help her fight heart failure and rhythm problems. Zocar was prescribed to enhance weight loss and help in the prevention of cardiovascular diseases (National heart lung and blood institute, 2011). For hypertension, Lasix was prescribed to treat her blood pressure problem and help her system get things back on track. Minipress Was given to treat high blood pressure and enhance the relaxation of the muscles around the urethra. Vasotec was administered together with other drugs to help in lowering of sallies blood

Keystone XL pipeline Essay Example | Topics and Well Written Essays - 500 words

Keystone XL pipeline - Essay Example â€Å"351 major infrastructure projects in the United States are caught up in regulatory or public acceptance hurdles (sometime the former triggered by the latter), including almost every kind of energy infrastructure imaginable from coal mining to transmission lines to solar or wind power generation facilities† (Ladislaw and Pumphrey). Besides, even if the project is rejected, the problem is not over as America still has to deal with a lot of issues. However, if it is accepted, it will give a boost to the American economy. The Perryman Group is a company of economic analysts in the USA who conducted an independent study and found that the construction of the Keystone XL pipeline would create an estimated 50000 spin-off and construction jobs which would range for at least a year in Texas alone. In addition to that, the construction of the Keystone XL pipeline would create many opportunities of employment indirectly. Above 250000 permanent jobs for Americans would be created by a permanent increase in the supply of oil. The annual expenditures in the USA would be increased by US$100 billion (Minicucci). What further justifies the US reliance upon the oil reserves in Canada is the fact that there are some two trillion barrels of oil in the sands in Canada which is eight times as much oil as is found in the reserves in Saudi Arabia (Minicucci). The Alberta oilsands are a huge source of jobs in Canada. More than 140,000 jobs can be attributed to the Alberta oilsands. Thus, approval of the construction of the Keystone XL pipeline would break the USA free of its economic dependency upon the Middle East to a large extent. Environmentalists have opposed the construction of the Keystone XL pipeline because it would prove a potential risk to a huge aquifer. There is a lot of subjectivity in the estimated risks. The environmentalists are â€Å"hell-bent on stopping the pipeline and, as a result, keeping the nation’s

Emaar Properties PJSC Coursework Example | Topics and Well Written Essays - 4000 words

Emaar Properties PJSC - Coursework Example Working Capital ratios 8 Investor performance 9 Conclusion 15 Bibliography 17 Executive Summary This report is a financial analysis of Emaar Properties PJSC which is one of the world’s largest and fastest growing real estate developers. Their performance is indicative of the huge rise in demand of living solutions for people around the globe. Emaar properties' has held a growing position in the industry as compared over the period of two years. The reports highlights on what financial effect the global financial crisis had on Emaar Properties’ performance. The continuous growth and expansion in various developments by Emaar Properties has played a vital role in its success. It has been competing well in its sector and industry within the geographical boundaries of Middle-East and has spread to international horizons as well. The leap to other countries has made Emaar Properties a global enterprise in the world of real-estate developers leading with innovations and state of the art facilities The report is clearly spread out in different sections according to financial results obtained while comparing Emaar from the year 2009 to 2010 and Emaar as compared to the industry average and its main competitors. This report is divided into parts and properly analyzed into different parts. The ratios have been calculated and analyzed as per the past record of Emaar properties. Other than that Emaar Properties figures have been compared to the Industry average too as well as other competitors. Emaar has been a leading real-estate developer and due to much expansion in its projects it has been retaining profits since 2007. It has been giving out zero dividends and all the money is invested back in the company. (Rasmala, 2010) Introduction Company Overview Emaar is one of the largest property investment organizations listed on the Dubai Financial Market (DFM) in the year 2000. It was started in 1997 and is currently known as Emaar Properties Public Joint Stock Limited Company (PJSC). Emaar offers high quality apartments to homebuyers with its full range to cater to their particular requirements. Emaar is innovating at a quick pace to provide premium lifestyle to its consumers globally. It is responsible for developing value-added, master planned communities along with homes. It has changed the concept of living lifestyle with its innovative designs and devel opment. Along with developing and expanding in the Dubai Market, it has reached out globally in various countries for future projects and has been active for quite some years now. It is expanding globally by spreading its vision of innovative lifestyle development and a new concept of ‘home’. Additionally Emaar is listed on two additional markets of Jones Arabia Titans Index and S&P IFCG Extended Frontier 150 Index. Emaar was also awarded the 462nd position in the world by 11th Financial Times Global 500 in 2007. (Emaar Properties, 2008) Financial Overview In June 2004, Emaar took the initiative of reducing the value of their shares from AED 10 to AED 1. It was done primarily to protect the interests of the shareholders and make their shares affordable for potential investors. Emaar further went on to do a 1:1 rights issue to double its capital in July 2005. (Emaar Properties, 2008) Ownership and Management Emaar is currently run by Mohamed Ali Alabbar who is the Direct or General of Dubai Department of Economics Development and also the Chairman of Emaar Properties. Emaar was privately held when it was established in 2007. After expanding into 60 different areas of development by its companies, it was listed in the Dubai Financial Markets. By 2007, the government of Dubai bought a 32% stake by giving the private investors AED28 billion worth of stock. The company also owns the Gold and Diamond Park in

Market Orientation Essay Example | Topics and Well Written Essays - 750 words

Market Orientation - Essay Example In such cases, marketing and advertising should be based on strategy different from the traditional approach (Mullins & Walker, 2010). Considering the case of funeral services, a business proposition that has gained widespread acceptance worldwide, still seems to be a taboo when it comes to marketing and advertising. Although human beings acknowledge the fact that death is inevitable, no one would eagerly wait or plan for it in advance, or even think about death. The Federal Trade Commission (2004) defines funeral services as services used to care for and prepare bodies for burial, cremation, or other final disposition; and, services used to arrange, supervise, or conduct the funeral ceremony or final disposition of human remains (Complying with the funeral rule, 2004; p.2). Without marketing the services, organizations providing these services will not be able to survive. However, marketing and advertising these services is very difficult as it might seem offensive to the general pu blic. Moreover, in this business, services are sought only when the need arises. The need is accompanied by many other deeper emotions, which could potentially suppress the buyers’ choice of company. This would again mean loss for the firms that are unable to spread awareness of their existence in the appropriate manner. In marketing sphere, advertising such products/services are commonly referred to as controversial advertising as they deal with socially sensitive issues. Such services have legal restrictions on advertising and portrayal. Waller (1999) points out that these products are faced with reasons related to delicacy, decency, morality, or even fear of distaste, disgust, offence, or outrage when mentioned or openly presented (p.289). In spite of these challenges, such services can be sold by adopting distant approaches. Advertising about provision of funeral services can be done through brochures, banners, posters, audio etc. Although it is good idea to make these ad vertisements impersonal or rather only information providing medium, it would be a better idea to make points that touch human emotions in the right manner. These advertisements must be more empathetic than sympathetic. In this sense, cognitive positioning that addresses the problems or solutions of the situation in which these services may be needed would give an empathetic perspective. To market about funeral services, the business owners cannot expect potential customers to walk up to them or start looking out for one. Hence, it becomes the onus of the business personnel to reach out to people where they can find potential for usage of their services. These could be hospice care centers, rehabilitation centers, florists, home for the elderly etc. When marketing or selling funeral services, it is important to establish a position as trusted partner who can assist the clients in the right manner in time of great need. By providing free information, funeral services can at least spr ead the awareness of their presence and their services which can be used when the need arises. The way this information is spread makes a lot of difference to the business. For example, when spreading information through brochures, video, audio etc, it is important to emphasize the name, logo, brad of the company and keep the information related to service more subtle in order to avoid sending out messages that might offend the public.

Discuss The Controversy Surrounding The Chapman Brothers Rectified Essay

Discuss The Controversy Surrounding The Chapman Brothers Rectified Works of Francisco Goyas Disasters of War Series - Essay Example The best in Goya came during the period of war in Spain and this shows that wars really bring out the best in men. The controversy that surrounded the rectification of his work by the Goya Brothers was borne out of the love that this critics had for this great artist and his art work. The Chapman brothers have been accused of defacing the etchings they purchased from Goya’s Disasters of War as the Chapman Brothers’ Insult to Injury had some funny faces that made their collections controversial in nature. The Brothers have been accused of intentionally defacing the appearance of Goya’s Disasters of War and some have even said that they used their Insult to Injury to mock Goya’s works. Although the Chapman Brothers have claimed that Francisco Goya was their model, the fact however remains that the rectification of his Disasters of War actually disproves this notion if one considers the fact that they tried to transform the work of their so called ‘mode l’ in order to produce their own work that is different from his work in several ways (Chapman). The Chapman Brothers succeeded in transforming Goya’s work into sheer pornographic Surrealism but it should be underscored that Goya was more into Romanticism. The issue that has been raised by the critics is that the Chapman Brothers bought a set of 83 etchings from Goya’s Disasters of War and vandalised it. The critics have claimed that the Chapman Brothers were aware that this great art work by Goya is one of the most venerated series of prints in existence (Mey, 2007, p.1). The Chapman Brothers have not only made attempts to erase the memories of the artists from the hearts of men, but they have also destroyed and obscured a great artwork, thus attracting lots of controversies to themselves and their rectified art work. Controversy based on Semiotics The Chapman Brothers unscrupulously rectified the works of Francisco Goya’s Disaster of War Series, which G oya himself used to represent the horrors of the war between 1810 and 1820. In the original work of Goya, he used symbols of humans as an allegory to the sufferings of humans during the war and to express his dissatisfaction and horror at the disgusting lunacy of war. In rectifying the works of Francisco Goya’s Disaster of War Series, the Chapman Brothers used cartoon images to represent the sufferings of humans that Goya was trying to depict through his images and the fact that Chapman derailed from the original message of Goya made people to criticize the revised works of the Chapman Brothers. â€Å"Typical in their broad-brush detestation of Enlightenment tradition are Jake and Dinos Chapman, postmodern artists most famous for ‘Insult to Injury’, their vandalized or ‘rectified’ appropriation of Goya’s Disaster of War prints, in which they pasted fiendish clown and puppy heads over every victim’s grimace....† (Broderick, 2005, p.9). The controversy that surrounded the works of the Chapman Brothers was caused by the cartoon images that they superimposed in their work as they used the symbols of puppies or clowns as figures to represent the human sufferings that Goya intended to show people through his Disaster of War

Compare and contrast utopian societies Research Paper

Compare and contrast utopian societies - Research Paper Example The Enlightenment was a remarkable time in human history. For many years, humans had lived in an intellectual or cultural â€Å"dark ages† where very little changed and people were wedded to their superstitions. Centuries went by and nothing really progressed. Instead of testing the world around them they simply accepted what clergymen or monarchs told them was true and left it at that. They didn’t test their limits; they just read old books and believed the facts in them. But this state of affairs could not last forever. There is an impulse, a curiosity, in humans that seeks sensible explanations. In the 17th century the Enlightenment began. Motivated by trade, the printing press, and a number of very significant intellectual leaders, this period of history saw a lot of the superstitions that guided people’s lives beaten back. Thinkers like Diderot, Voltaire, Adam Smith, and Thomas Jefferson revolutionized the way we think about the world and our place in it. Sc ientific innovation was also telling us more and more about our world, was exposing the fact that it was not run by ghosts and gods. Things began to change dramatically. People believed that a better world could be created through reason. The first utopians became famous. The power of science was very important to the Enlightenment and to its idea that utopias were possible. Science was the process people used to explain the world to themselves and it was really coming into its own. People like Galileo had shown us that the moon was not a perfect sphere and that the patterns of the other planets were not quite as perfect as once thought. People began to think that the world was knowable not by divine revelation but through scientific reasoning and measurement. They took up their slide rules and went to their labs to try to explain things. This was a positive thing. But some people also began to applying strict science to things like politics or race—concepts that

Discuss The Controversy Surrounding The Chapman Brothers Rectified Essay

Discuss The Controversy Surrounding The Chapman Brothers Rectified Works of Francisco Goyas Disasters of War Series - Essay Example The best in Goya came during the period of war in Spain and this shows that wars really bring out the best in men. The controversy that surrounded the rectification of his work by the Goya Brothers was borne out of the love that this critics had for this great artist and his art work. The Chapman brothers have been accused of defacing the etchings they purchased from Goya’s Disasters of War as the Chapman Brothers’ Insult to Injury had some funny faces that made their collections controversial in nature. The Brothers have been accused of intentionally defacing the appearance of Goya’s Disasters of War and some have even said that they used their Insult to Injury to mock Goya’s works. Although the Chapman Brothers have claimed that Francisco Goya was their model, the fact however remains that the rectification of his Disasters of War actually disproves this notion if one considers the fact that they tried to transform the work of their so called ‘mode l’ in order to produce their own work that is different from his work in several ways (Chapman). The Chapman Brothers succeeded in transforming Goya’s work into sheer pornographic Surrealism but it should be underscored that Goya was more into Romanticism. The issue that has been raised by the critics is that the Chapman Brothers bought a set of 83 etchings from Goya’s Disasters of War and vandalised it. The critics have claimed that the Chapman Brothers were aware that this great art work by Goya is one of the most venerated series of prints in existence (Mey, 2007, p.1). The Chapman Brothers have not only made attempts to erase the memories of the artists from the hearts of men, but they have also destroyed and obscured a great artwork, thus attracting lots of controversies to themselves and their rectified art work. Controversy based on Semiotics The Chapman Brothers unscrupulously rectified the works of Francisco Goya’s Disaster of War Series, which G oya himself used to represent the horrors of the war between 1810 and 1820. In the original work of Goya, he used symbols of humans as an allegory to the sufferings of humans during the war and to express his dissatisfaction and horror at the disgusting lunacy of war. In rectifying the works of Francisco Goya’s Disaster of War Series, the Chapman Brothers used cartoon images to represent the sufferings of humans that Goya was trying to depict through his images and the fact that Chapman derailed from the original message of Goya made people to criticize the revised works of the Chapman Brothers. â€Å"Typical in their broad-brush detestation of Enlightenment tradition are Jake and Dinos Chapman, postmodern artists most famous for ‘Insult to Injury’, their vandalized or ‘rectified’ appropriation of Goya’s Disaster of War prints, in which they pasted fiendish clown and puppy heads over every victim’s grimace....† (Broderick, 2005, p.9). The controversy that surrounded the works of the Chapman Brothers was caused by the cartoon images that they superimposed in their work as they used the symbols of puppies or clowns as figures to represent the human sufferings that Goya intended to show people through his Disaster of War

In writing a narrative essay Essay Example for Free

In writing a narrative essay Essay Include sensory and emotional details, so the reader will experience the story, not just read about it Have the story support the point you are making, and make reference to that point in the first sentence. Write in the first or third person Descriptive Descriptive essays have text which describes traits and characteristics of people, objects, events, feelings, etc in intricate detail. Whatever is being described will be thoroughly examined. For example, if you were describing roses, you would explain: Where they come from What they look like What colors they are How they grow and smell When you write a descriptive essay, you want to involve the reader’s senses and emotions. For example, you could say, â€Å"I got sleepy† or describe it like this, As I was waiting for Santa, my eyelids began to get heavy, the lights on the tree began to blur with the green branches, and my head started to drop. The second sentence gives vivid details to make the reader feel like he is there. Exposition See more: how to start a personal narrative essay examples Expository essays can compare, explore and discuss problems, or tell a story. An exposition essay gives information about various topics to the reader. It: Informs Describes Explains In writing an exposition, the text needs to: Be concise and easy to understand Give different views on a subject or report on a situation or event Explain something that may be difficult to understand as you write your essay. Remember that your purpose is to explain. Argumentative In an argumentative essay the writer is trying to convince the reader by demonstrating the truth or falsity of a topic. The writer’s position will be backed up with certain kinds of evidence, like statistics or opinions of experts. The writer is not just giving an opinion, but making an argument for or against something and supporting that argument with data. To know how to write an essay in an argumentative way, you have to research and backup what you say in the text. Learn by Example When learning how to write an essay, sometimes the best way to learn is to look and analyze essay examples. Following are excerpts from narrative essays: Looking back on a childhood filled with events and memories, I find it rather difficult to pick on that leaves me with the fabled warm and fuzzy feelings. As the daughter of an Air Force Major, I had the pleasure of traveling across America in many moving trips. I have visited the monstrous trees of the Sequoia National Forest, stood on the edge of the Grande Canyon and have jumped on the beds at Caesar’s Palace in Lake Tahoe. The day I picked my dog up from the pound was one of the happiest days of both of our lives. I had gone to the pound just a week earlier with the idea that I would just look at a puppy. Of course, you can no more just look at those squiggling little faces so filled with hope and joy than you can stop the sun from setting in the morning. I knew within minutes of walking in the door that I would get a puppy†¦ but it wasnt until I saw him that I knew I had found my puppy. Looking for houses was supposed to be a fun and exciting process. Unfortunately, none of the ones that we saw seemed to match the specifications that we had established. They were too small, too impersonal, too close to the neighbors. After days of finding nothing even close, we began to wonder: was there really a perfect house out there for us? The following is an example of a famous narrative written by John Updike, Hub Fans Bid Kid Adieu: The afternoon grew so glowering that in the sixth inning the arc lights were turned onalways a wan sight in the daytime, like the burning headlights of a funeral procession. Aided by the gloom, Fisher was slicing through the Sox rookies, and Williams did not come to bat in the seventh. He was second up in the eighth. This was almost certainly his last time to come to the plate in Fenway Park, and instead of merely cheering, as we had at his three previous appearances, we stood, all of us, and applauded. Following are excerpts from descriptive essays: Like his twisted feathers, his many scars, the reliable old owl chose the gnarled, weather-beaten, but solid branch often—it being a companion to the wise alone with the night and the last branch to creak in the heaviest wind. He often came to survey the fields and the clouds before his hunt, to listen to the steady sound of the stream passing through reeds under the bridge, while combing his feathers for the unwanteds—whatever they might be. Here is a descriptive essay about a first visit to a favorite diner written by a student at Roane State Community College:When entering the door at Lou’s, two things are immediately noticeable: the place is rarely empty and seems to consist of a maze of rooms. The first room, through the door, is the main part of the restaurant. There is another, rarely used, dining room off to the right. It was added during the oil well boom of the seventies. Through the main dining room is yet another room; it guards the door leading into the kitchen. This room contains the most coveted table in the place. The highest tribute Lou can bestow on anyone is to allow them access to seats at this table. This table is the family table; it is reserved for Lou’s, and her daughter Karen’s, immediate family and treasured friends. Here is an example of a descriptive essay from St. Cloud State:Billy Rays Pawn Shop and Lawn Mower Repair looked like a burial ground for country auction rejects. The blazing, red, diesel fuel tanks beamed in front of the station, looking like cheap lipstick against the pallid, wrinkled texture of the parking lot sand. The yard, not much larger than the end zone at General G. Patton High School on the north end of town, was framed with a rusted metallic hedge of lawn mowers, banana seat bicycles, and corroded oil drums. It wasnt a calico frame of rusted parts, but rather an orchestra of unwanted machinery that Billy Ray had arranged into sections. The yellow-tanked mowers rested silently at the right of the diesel fuel. Once red, now faded orange, mowers stood at attention to the left. The oil barrels, jaded and pierced with holes, bellared like chimes when the wind was right. The bikes rested sporadically throughout the lot. In the middle of it all was the office, a faded, steel roof supported by cheap two-by-fours and zebra paneling. Billy Ray was at home, usually, five blocks east of town on Kennel Road. Following are excerpts from exposition essays: This family was a victim of a problem they could have avoided-a problem that, according to Florida park rangers, hundreds of visitors suffer each year. Several times a month, ranger Rod Torres of OLeno State Park said, people get scared and leave the park in the middle of the night. Those people picked the wrong kind of park to visit. Not that there was anything wrong with the park: The hikers camped next to them loved the wild isolation of it. But it just wasnt the kind of place the couple from New Jersey had in mind when they decided to camp out on this trip through Florida. Here is an example of a student model answer of an Expository Essay from The Write Source:Did you know that 7 out of 10 students have cheated at least once in the past year? Did you know that 50 percent of those students have cheated more than twice? These shocking statistics are from a survey of 9,000 U.S. high school students.Incredibly, teachers may even be encouraging their students to cheat! Last year at a school in Detroit, teachers allegedly provided their students with answers to statewide standard tests.Here is an another example of an expository essay. This example comes from Essay Start:Throughout history and through a cross-section of cultures, women have transformed their appearance to conform to a beauty ideal. Ancient Chinese aristocrats bound their feet as a show of femininity; American and European women in the 1800s cinched in their waists so tightly, some suffered internal damage; in some African cultures women continue to wear plates in their lower lips, continually stretching the skin to receive plates of larger size. The North American ideal of beauty has continually focussed on womens bodies: the tiny waist of the Victorian period, the boyish figure in vogue during the flapper era, and the voluptuous curves that were the measure of beauty between the 1930s and 1950s. Current standards emphasize a toned, slender look, one that exudes fitness, youth, and health. According to psychologist Eva Szekely, Having to be attractive at this time . . . means unequivocally having to be thin. In North America today, thinness is a precondition for being perceived by others and oneself as healthy (19). However, this relentless pursuit of thinness is not just an example of women trying to look their best, it is also a struggle for control, acceptance and success. Finally, here are excerpts from argumentative essays: Gun control has been a controversial issue for years. A vast majority of citizens believe that if gun control is strictly enforced it would quickly reduce the threat of crime. Many innocent people feel they have the right to bear arms for protection, or even for the pleasure of hunting. These people are penalized for protecting their lives, or even for enjoying a common, innocent sport. To enforce gun control throughout the nation means violating a persons Constitutional rights. Although some people feel that the issue of gun control will limit crime, the issue should not exist due to the fact that guns are necessary for self defense against crime, and by enforcing gun control is violating a citizen’s second amendment right to bear arms. Another examples of an argumentative essay comes from Bogazici University:Throw out the bottles and boxes of drugs in your house. A new theory suggests that medicine could be bad for your health, which should at leastcome as good news to people who cannot afford to buy expensive medicine. However, it is a blow to the medicine industry, and an evenbigger blow to our confidence in the progress of science. This new theory argues that healing is at our fingertips: we can be healthy by doing Reikion on a regular basis. On Essay By Example, on the other hand, the sample argumentative essay addresses online games and socialization:

Recombinant Green Fluorescent Protein Purification

Recombinant Green Fluorescent Protein Purification A series of experiments were performed on the E.coli strain BL21 pLysS pRSETA-GFPUV in order to express and purify a recombinant form of Green Fluorescent Protein (rGFP) using Ni2+-Agarose chromatography based on the rGFP His6 tag properties. A rGFP crude extract (GCE) was collected and later purified resulting in 10 washes and 10 elutions. A Bradford assay was performed on the first 6 samples of the washes and elutions to determine activity via relative fluorescent units (RFUs). A sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-Page) followed to determine purity of the samples and a Western Blot verified the presence of rGFP. The sample with the highest activity was the E3 having 31,927 RFUs with an estimated purity of 10 percent and a molecular weight of 36 kDa. The estimated total yield of our protein is 2.07 ug. Introduction First discovered by Osamu Shimomura in 1961, the Green Fluorescent Protein (GFP) was isolated and identified as a protein that fluoresce green light. When calcium binds to the photoprotein aequorin, in vitro aequorin produces blue light (1). However, in the original jelleyfish A. equorea victoria green light was produced. The green light produced was the result of a second protein GFP driving its excitation energy from aequorin (2). After purification, GFP is a protein of 238 amino acids absorbing blue light at 395 nm and emitting green light at nearly 509 nm (2, 3, 4). This chromophore is very stable towards multiple harsh conditions including extreme pH and heat (5). The Aequorea GFP also measures to be at 30 kDa monomer (6). Purifying rGFP required a unique way of identifying the protein without isolating other protein contaminants. This particular rGFP was tagged genetically to the N-terminal of the protein sequence. This tag is useful due to the unique property of the chromatography technique used. Using Ni2+ -agarose affinity chromatography, the histadine tagged rGFP binds to the Ni2+ , thereby attaching this particular protein to the column. Next, the rGFP is eluted from the Ni2+ -agarose column by running a competitor compound that has a higher affinity for the Ni2+ called imidazole. The His6 tag unbinds from the column allowing the rGFP protein to be collected for experimental purposes (7). The purpose of this experiment is to express and purify the E. coli strain BL21(DE3) using Ni2+ -agarose affinity chromatography followed by the SDS-PAGE and Western Blot procedures to estimate purity and confirmation of the protein. Materials and Methods Expression of rGFP and Preparation of the rGFP Crude Extract (GCE) The BL21(DE3) bacterial culture referred to as G was used to inoculate 10 ml liquid LB [100ug/ml Amp;25ug/ml Cam] growth media and grown overnight at 37 degrees celsius with vigorous shaking. OD600 of .1 of 500 ml of liquid LB growth media was achieved after a second inoculation was achieved with the culture grown overnight. The 500 ml culture was allowed to grow to OD600 reached .5 by vigorous shaking at 37 degrees celsius. The bacterial pellet was stored at -20 degrees celsius and labeled as G0 while 1 ml of the culture was pelleted in a centrifuge tube and induced with IPTG. At the time of induction the cultures relative time is zero. Three hours post induction, the culture was labeled G3, stored at -20 degrees celsius after the supernatant discarded. The same procedure was done to the G3-15 ml with the exception of pelleting 15 ml of the culture. Next, the culture was vortexed at 37 degrees celsius to lyse the bacteria. A 1 ml breaking buffer was added, solution vortexed, and placed in a 37 degrees celsius water bath. After the centrifugation the supernatant was decanted into a new tube labeled GCE representing rGFP crude extract. (8). Purification of rGFP using Ni2+-Agarose Affinity Chromatography A plastic syringe with a luer-lock was blocked by glass wool to hold in the Ni2+ Agarose matrix. The syringe was then secured vertically using a ring stand and filled with approximately 100ul of buffer followed by 2 ml to ensure the removal of air bubbles trapped in the system. A 50 % slurry of Ni2+-agarose was added to 500 ul buffer on top of the glass wool. The system is then opened to ensure packing of the agarose matrix towards the bottom. The final step in setting up the column is the pre-equilibration step which is the addition of breaking buffer to the column while the leur-lock is open until the ethanol is out of the system. After storing away 100 ul of GCE for future use, 1 ml of breaking buffer was added to the centrifuge tube. The GCE was transfered to the Ni2+ -agarose column. After a 10 minute period the luer-lock was opened and .5 ml effluent liquid was collected and labeled W1 followed by W2 until W10. Meanwhile washing the unbound proteins with 4 ml of breaking buffer. After the 10th wash was collected, the column was washed with an additional 5 ml of breaking buffer. A similar process was followed collecting elution 1 through 10 in 1.5 centrifuge tubes, however by adding the elution buffer containing imidazole. The elution buffer formula available in the solution manual (8). Estimating Protein Concentration of rGFP Determine protein amount using the Bradford assay requires a standard curve using known amount of Bovine Serum Albumin (BSA). The Bradford assay was performed on six known amounts of BSA (0, 2.5, 5,10, 15, and 20 ug). 50 ul of solution containing the BSA was added with 1 ml of Bradford reagent to a glass tube. The solution was mixed and incubated at room temperature for 10 minutes. 200 ul of the 6 assays were transferred to a microtiter dish to read the absorbance at 595 nm in a microplate reader. A standard curve was plotted (Absorbance Vs BSA amount) to determine the highest absorbance reading that can be extrapolated from this curve. The same procedure was done on the first six washes and six elutions in triplicate. (9). SDS-PAGE/Coomassie Blue Analysis procedure Two recipes were followed in order to make a resolving gel and a stacking gel. The 12 % resolving gel consists of water, 4x resolving buffer, 30 % Acylamide, 10% ammonium persulfate, and TEMED. This solution was poured between two glass plates until polymerization. The 5 % stacking gel consisted of water, 4x stacking buffer, 30 % Acrylamide, 10 % ammonium persulfate, and TEMED. The Stacking buffer was added on top of the resolving buffer followed by the addition of a toothed comb until polymerization. Afterwards, the samples G0, G3, GCE, W2, W3, E2, E3 were loaded into the gel. The loading of the samples was done after the plates were placed in the electrophoresis tank. The electrophoresis tank then ran for 45 minutes at 200 volts. (10). Preparation and Development of the Western Blot Using the 7 samples from the previous procedure, we add 2-Me (Beta-mercaptoethanol) to each sample and developed another gel. After electrophoresis, the gel was to be used as a part of a sandwich for the Western Blot. The sandwich consisted of (from the top) a clear cassette lid, sponge, filter paper, nitrocellulose, gel, filter paper, and sponge that laid against the black cassette lid. After an incubation period, the protein transferred from the gel to the nitrocellulose. Removing the nitrocellulose to a container, the Ponceau S stain was added for two minutes and rinsed several times with distilled water. This was done until red bands appeared. The molecular weight ladder was marked with pencil for further review. Next a blocking step followed where the nitrocellulose membrane was placed in a Tupperware with 30 ml of 5% non-fat dry milk/TBS solution. The Tupperware was placed on a shaking platform for 30 minutes. Afterwards, the blocking solution was discarded and a washing step compromising of 30ml of .05% Tween 20/TBS solution was added. The Tupperware was placed on a shaking platform for 5 minutes. This process was repeated two more times. Following the washing step a primary probing step was performed by adding 7 ml of mouse IgG anti-Xpress epitope MAb solution and incubated on a shaking platform for 45 minutes. Another washing step followed using 30 ml of .05% Tween 20/TBS and repeated two more times. 7 ml of Sheep IgG anti-mouse IgG conjugated horse radish peroxidase polyclonal anti-serum solution was added in a secondary probing step that lasted 45 minutes on a shaking platform. The same washing step previous done followed and repeated two times. Finally, the final wash step with 30 ml of TBS was performed on a shaking platform for 5 minutes. For the final step, 7 ml of TMB substrate solution was added to the membrane until band color intensity was achieved. Then the membrane was moved into a Tupperware container filled with water to stop the development. The nitrocellulose membrane was then dried and results recorded. (11). Results The bacterial expression system of rGFP is presented in Figure 1. The Lac repressor is made from Lac1 of the bacterial chromosome. The lac repressor blocks the t7 RNA polymerase but after inducing it with IPTG, the Lac repressor itself will be blocked. Hence, T7 RNA polymerase will start being abundant and be the promoter to the expression for the His6-Xpress-GFPuv thereby resulting in rGFP (7). For a better understanding of rGFP a schematic diagram is provided in Figure 2. The elution of interest was E3 which had 31,927, the highest relative fluorescent units. We also see that from the combined elution profile in Figure 3 which displays the RFU for the first six washes and elutions. The samples were then run through a Bradford assay. The E3 sample had a 20.7 +/- 12.45 ug total amount of protein. The specific activity was 342,995 RFU/mg. The SDS-PAGE gel (Figure 4) presented the molecular weight of E3 standing at 36 kDa. We determined that using the ladder provided. The results gathered was off by 3 kDa from the verified experimental value of the rGFP. From the relative color density, it was determined that the rGFP band retains 10 % purity which resulted in a calculated yeild of 2.07 ug. Figure 5 presents the Western Blot assay which was used to verify the presence of rGFP. E3 and E2 showed a stronger color while G0 as expected did not show a band due to lack of rGFP. The washes also show a faint color. We have confirmed the presence of rGFP by comapring the E3 band to the ladder which approximates 36 kDa. Conclusion/Discussion The confirmation of rGFP was obtained though proceeding with a Western blot analysis. The experiments that were performed beforehand gave a good understanding of how rGFP was induced, expressed, and purified. To recap, Ni2+-agarose affinity chromatography was used to isolate our protein through the unique property having affinity to the His6 tag in the rGFP. Followed by the Bradford assay we estimated how much protein the samples contained. The SDS-PAGe gel gave us an estimation of the molecular weight and purity of the samples which was paramount in the bigger picture of identifying the protein. Even though the purity gained was very low, we proceeded and developed a Western Blot which confirmed the presence of rGFP through band intensities. Since the GFP protein is very robust to pH and temperature, one can transfect or transcribe this gene into other living bacteria or even human cells to possibly see movement. One follow up experiment would to do just that, seeing if we can insert the rGFP into cancer cells or enzymes like insulin for further studies. We already know that GFP changes color based on the excitation energy which might be altered to produce different colors. This can be used to study two systems or their interactions or lack of interaction. We can study the energy consumption of different bacteria and learn which colonies survive longer. We can apply this method in cancer research and find out how cancer fast certain cancers grow by studying the relative fluorescence given off. The impact of this finding may be similar to the impact of creating spectacles (glasses) that allowed millions to see clearly. This protein offers that unique new ability to visually track things we could not have tracked as easily. References O. Shimomura, F. H. Johnson, Y. Saiga. J. Cell. Comp. Physiol. 59, 223 (1962). J. G. Morin and J. W. Hastings, J. Cell Physiol. 77, 313 (1971); H. Morise, O. Shimomura, F. H. Johnson, J. Winant, Biochemistry 13, 2656 (1974). D. C. Prasher, V. K. Eckenrode, W. W. Ward, F. G. Prendergast, M. J. Cormier, Gene 111, 229 (1992) . W. W. Ward, C. W. Cody, R. C. Hart, M. J. Cormier, Photochem. Photobiol. 31, 611 (1980). Ward, W.W. and Bokman, S.H.: Reversible denaturation of Aequorea green-fluorescent protein: physical separation and characterization of the renatured protein. Biochemistry 21 (1982) 4535-4550. Prendergast, F.G. and Mann, K.G.: Chemical and physical properties of aequorin and the green-fluorescent protein isolated from Aequorea forskalea. Biochemistry 17 (1978) 3448-3453. R. Scott, and E. Picket. Biochemistry Laboratory Manual. United States. (2012). R. Scott, and E. Picket. Biochemistry Laboratory Manual. United States. 84-88 (2012). R. Scott, and E. Picket. Biochemistry Laboratory Manual. United States. 99-100 (2012). R. Scott, and E. Picket. Biochemistry Laboratory Manual. United States. 125-126 (2012). R. Scott, and E. Picket. Biochemistry Laboratory Manual. United States. 139-140 (2012). R. Scott, and E. Picket. Biochemistry Laboratory Manual. United States. (2012). R. Scott, and E. Picket. Biochemistry Laboratory Manual. United States. (2012). citations: Primary stucture of the aequorea victoria GRP Douglas prasher, virginia eckenrode-229-223 1992 GFP as a marker for gene expression martin chalfie, vuan tu vol 263, feb 1994 Wavelength mutations and post translational autoxidation of GFP vol 91, pp. 12501-1250 dec 1994